母马低剂量组氨瑞林诱导排卵：一项部分随机交叉预试验。
完整摘要 · 中文译文
这项部分随机交叉先导研究评估了减少剂量的组氨瑞林用于母马诱导排卵的效果，并比较了肌肉注射（IM）给药与在白会和雁翅穴位进行药物穴位注射的差异。共纳入11匹母马，其中9匹完成了全部八个方案并被纳入分析。方案包括250 µg组氨瑞林IM（标准剂量）、75 µg IM、25 µg IM、无干预、白会穴注射生理盐水、雁翅穴注射生理盐水、白会穴注射25 µg组氨瑞林以及雁翅穴注射25 µg组氨瑞林。排卵通过经直肠超声检查在预定时间点进行评估，直至检测到排卵，主要结局为48 h内排卵。首次在24 h检测到的排卵被排除在主要分析之外。48 h内排卵发生在250 µg IM的88.9%（8/9）观察中、75 µg IM的87.5%（7/8）、25 µg IM的87.5%（7/8）、无干预的37.5%（3/8）、白会穴注射生理盐水的50.0%（3/6）、白会穴注射25 µg组氨瑞林的100%（9/9）、雁翅穴注射生理盐水的12.5%（1/8）以及雁翅穴注射25 µg组氨瑞林的87.5%（7/8）中。精确95%置信区间较宽，反映了样本量较小。与无干预方案的探索性两两比较未对多重比较进行校正，应谨慎解读。这些发现提供了初步证据，表明减少剂量的组氨瑞林，包括25 µg，可能在母马中48 h内诱导排卵。然而，本研究未证明药物穴位注射相对于相同减少剂量肌内注射具有优势。需要更大规模、完全随机化的研究。 还需要进一步研究来证实减量组氨瑞林的有效性，并确定在特定穴位给药是否能提供额外的效果。
完整摘要 · 英文原文
This partially randomized crossover pilot study evaluated reduced doses of histrelin for ovulation induction in mares and compared intramuscular (IM) administration with pharmacopuncture at the Bai-hui and Yan-chi acupoints. Eleven mares were enrolled, of which nine completed all eight protocols and were included in the analyses. The protocols comprised 250 µg histrelin IM (standard dose), 75 µg IM, 25 µg IM, no intervention, saline at Bai-hui, saline at Yan-chi, 25 µg histrelin at Bai-hui, and 25 µg histrelin at Yan-chi. Ovulation was assessed by transrectal ultrasonography at scheduled examinations until detected, with ovulation within 48 h as the primary outcome. Ovulations first detected at 24 h were excluded from the primary analysis. Ovulation within 48 h occurred in 88.9% (8/9) of observations with 250 µg IM, 87.5% (7/8) with 75 µg IM, 87.5% (7/8) with 25 µg IM, 37.5% (3/8) with no intervention, 50.0% (3/6) with saline at Bai-hui, 100% (9/9) with 25 µg histrelin at Bai-hui, 12.5% (1/8) with saline at Yan-chi, and 87.5% (7/8) with 25 µg histrelin at Yan-chi. Exact 95% confidence intervals were wide, reflecting the small sample size. Exploratory pairwise comparisons with the no-intervention protocol were performed without adjustment for multiple comparisons and should be interpreted cautiously. The findings provide preliminary evidence that reduced histrelin doses, including 25 µg, may induce ovulation within 48 h in mares. However, this study did not demonstrate an advantage of pharmacopuncture over IM administration of the same reduced dose. Larger, fully randomized studies are required to confirm the efficacy of reduced-dose histrelin and determine whether administration at specific acupoints provides any additional effect.